| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187534 |
| Chromosome: | chromosome 6 |
| Location: | 2973450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273300 | TST1 | (1 of 2) 6.1.1.3 - Threonine--tRNA ligase / Threonyl-tRNA synthetase; Threonyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATAGGCGCGCGCGAGTGCTGTACAGGGG |
| Internal bar code: | GGGTGCCGCGCAACGCAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.6896 |
| LEAP-Seq n confirming: | 8672 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCAATCCCAAATGGAC |
| Suggested primer 2: | ACAAGAGCGGCTCAGAAGAG |