| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187539 |
| Chromosome: | chromosome 9 |
| Location: | 6568459 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408100 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGAGTTGTGCAATGTCGGCCCGGGTC |
| Internal bar code: | CATAAAGTTGGTGCGTGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 99.8448 |
| LEAP-Seq n confirming: | 1287 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGTGCAAGCGCAAATGTC |
| Suggested primer 2: | ACCCTCTCAAACCCACACAC |