| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187548 |
| Chromosome: | chromosome_12 |
| Location: | 5288609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g528850 | DLX1,DLC3 | Flagellar outer dynein arm 16 kDa light chain, LC3 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCCTCGCTGGGTCTATCCCTCAGGGTT |
| Internal bar code: | ATGAGTAACGTTCTACGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 98.5014 |
| LEAP-Seq n confirming: | 723 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCACAGGGCATCACTA |
| Suggested primer 2: | AGCAGCTATGGGCTGACTGT |