Insertion junction: LMJ.RY0402.187605_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187605
Chromosome:chromosome 6
Location:5148255
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g281350 LON1 (1 of 1) K08675 - Lon-like ATP-dependent protease [EC:3.4.21.-] (PRSS15, PIM1); Mitochondrial LON protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAGGGAGAGGGAAGGGGGAGGGGCTGGT
Internal bar code:GGCAAGAACGATGCCGAGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:235
LEAP-Seq percent confirming:46.3918
LEAP-Seq n confirming:270
LEAP-Seq n nonconfirming:312
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCTCGTTTTAGCTTGTT
Suggested primer 2:CCCTCACAGTCAAAAACCGT