Insertion junction: LMJ.RY0402.187611_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.187611
Chromosome:chromosome 3
Location:1026705
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g148450 TEH1 Acetyl-CoA thioesterase/hydrolase; (1 of 1) 3.1.2.1 - Acetyl-CoA hydrolase / Acetyl-CoA deacylase 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGAGACAATCTTCTCGGAACACCCTT
Internal bar code:ACGCGGATGGGGTCCGGTGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:210
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTAGCTTCGGGACTTTC
Suggested primer 2:ATTACGATACGTTGAGCCCG