| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187645 |
| Chromosome: | chromosome 2 |
| Location: | 5209476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105800 | (1 of 2) PTHR24128:SF17 - E3 UBIQUITIN-PROTEIN LIGASE XBAT32 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTGGGCGGTGCTGCACAGGCTGCGAA |
| Internal bar code: | GTGCGTGGCACCCGGAACGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 88.8298 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTGCAGGTACCCTCTC |
| Suggested primer 2: | TAGCCTGTGTGATGCTGGAG |