| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187675 |
| Chromosome: | chromosome_6 |
| Location: | 5593902 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g285600 | RWP5,MID | RWP-RK transcription factor/minus dominance protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGACACCAGCTGGCCAACACCCCTCGCC |
| Internal bar code: | GCGAAGGCGTACGCGGGGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 99.9053 |
| LEAP-Seq n confirming: | 1055 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGGACTGCAACTCAGCG |
| Suggested primer 2: | CGCATCAAGCTGATACTGGA |