| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187683 |
| Chromosome: | chromosome_9 |
| Location: | 3661440 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390578 | FAP11 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTCCAAAGTACATGGCCCACGACATGCCC |
| Internal bar code: | GCAGGACAATGGAGCCGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.3007 |
| LEAP-Seq n confirming: | 852 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGCAGGAAGTTCTGTG |
| Suggested primer 2: | ACCATGTTCGACGTGTTTGA |