| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187689 |
| Chromosome: | chromosome 7 |
| Location: | 256288 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313950 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGTCTAGAGCACAAACACTCGGCCCG |
| Internal bar code: | CGCTGGGTTTCGACCGCAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 99.6538 |
| LEAP-Seq n confirming: | 1727 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCACAGAAAGGTGCGAT |
| Suggested primer 2: | TGAAGACGCGATTACGACAG |