| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187704 |
| Chromosome: | chromosome 7 |
| Location: | 1828633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325729 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCGGAAGGTGCGTACAGGTCCGGTGT |
| Internal bar code: | GGTTGATTGCGCATACATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 98.8642 |
| LEAP-Seq n confirming: | 4091 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACAGAATAGCCACAGCG |
| Suggested primer 2: | GTGGCAGACAGCGAAGTACA |