Insertion junction: LMJ.RY0402.187727_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.187727
Chromosome:chromosome 1
Location:5225826
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g036350 PMA1 (1 of 5) K01537 - Ca2+-transporting ATPase (E3.6.3.8); Related to plasma membrane P-type and chloroplast type calcium ATPases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGCCGCTGCTGGCGCTGGTGCTACAGC
Internal bar code:ACTAGGCAGTTAGAGTATGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:1111
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTATCATCGAGGTGGTGG
Suggested primer 2:AGGTCTGTGTTTCGGGAATG