| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187743 |
| Chromosome: | chromosome 4 |
| Location: | 4082759 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233302 | (1 of 2) PTHR23132//PTHR23132:SF9 - D-ALANINE--D-ALANINE LIGASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCCTCCCGACACAGCGCACCTTCGCG |
| Internal bar code: | GCCTGTTCAAGTTCGGACCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1156 |
| LEAP-Seq percent confirming: | 99.8783 |
| LEAP-Seq n confirming: | 821 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTCAATTCTCGCAGGC |
| Suggested primer 2: | CGTACACGACAACCTTCCCT |