| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187748 |
| Chromosome: | chromosome 7 |
| Location: | 3894054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339100 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGCGGACGGAGGTAATAACGGACTTA |
| Internal bar code: | CGCAAGGAACCACATGAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 99.7783 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGGTAGGTGGGTAGGT |
| Suggested primer 2: | ACCAAAACATCACCAGCTCC |