| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187751 |
| Chromosome: | chromosome 15 |
| Location: | 686759 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640350 | CLR4,OPR97,NCL4 | Nuclear Control of chloroplast Like 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGTGCATGCGTTGGAGTTTGGCAGACG |
| Internal bar code: | TAGGTATATCGGGCGCTCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 98.3478 |
| LEAP-Seq n confirming: | 2381 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGAGTGAAGCCTTTCAA |
| Suggested primer 2: | AGGCAGTGCAGAGGTTGTTT |