| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187765 |
| Chromosome: | chromosome 11 |
| Location: | 901732 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467656 | (1 of 9) IPR007112//IPR009009 - Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCATCTATCCCCATCCAAACACCCAC |
| Internal bar code: | ACAACGTGGCTGCGCGTATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 98.8604 |
| LEAP-Seq n confirming: | 1041 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAAACCCGCAAACTGAC |
| Suggested primer 2: | CGTGTGTGTGTTTTCCCTTG |