| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187789 |
| Chromosome: | chromosome 17 |
| Location: | 3740300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACCGCCGCATGTCAACAGCTAGCATGC |
| Internal bar code: | GTTGTGTCAGATGAGAGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 99.8273 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTATACATAGCCAGGCGT |
| Suggested primer 2: | AGATGCTGGGCAACCACTAC |