Insertion junction: LMJ.RY0402.187816_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.187816
Chromosome:chromosome 2
Location:1598964
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g085050 COP1,PCOP1 Plant-type constitutive photomorphogenesis protein 1; (1 of 2) K10143 - E3 ubiquitin-protein ligase RFWD2 (RFWD2, COP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGATGCTGTACCAAATATTTCCGCGTT
Internal bar code:GCGGATACCAGGGGTACAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:83.2514
LEAP-Seq n confirming:1864
LEAP-Seq n nonconfirming:375
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAGTTGTCACCTTGAGT
Suggested primer 2:CGGTGTCTTTGGTGGTCTTT