| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187824 |
| Chromosome: | chromosome 9 |
| Location: | 946259 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400950 | NCT2 | (1 of 2) PTHR11662:SF3 - INNER MEMBRANE TRANSPORT PROTEIN RHMT; High-affinity nicotinic acid transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGCCTTCGCCGCCATCCAGCTCAACG |
| Internal bar code: | CTCAGTCGCCCCGCGTGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 8.6166 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 1156 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGTTGTGCTAGTGTGGC |
| Suggested primer 2: | CCCAGGCGAATATGATGAAC |