| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187853 |
| Chromosome: | chromosome 3 |
| Location: | 8838182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206705 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACCAGCACTCCATCCCGATCCATCCCA |
| Internal bar code: | ATTCCGCATTGGCTGACAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.8878 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCCACTCCATTTTCTCC |
| Suggested primer 2: | CGTGTCACGCATAACAGTCC |