| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187866 |
| Chromosome: | chromosome 10 |
| Location: | 4228083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450100 | (1 of 1) K04957 - hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACTCAGACCAAGTGATCACGCCCGCA |
| Internal bar code: | CTGGGATGGCGCGGACGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.7455 |
| LEAP-Seq n confirming: | 784 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATCTGGCGTGACTAC |
| Suggested primer 2: | GACGAAACCATAACGCAGGT |