| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187871 |
| Chromosome: | chromosome 10 |
| Location: | 3978981 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448700 | (1 of 1) K11650 - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D (SMARCD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTATAAAATGCGCATGTAATACTTATC |
| Internal bar code: | GCATGCGGCTTCTTTAGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 99.8163 |
| LEAP-Seq n confirming: | 1087 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAGATCCAACAGGTGGT |
| Suggested primer 2: | TGTGTCGCCATTGAGCTTAG |