| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187901 |
| Chromosome: | chromosome 2 |
| Location: | 2503792 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092150 | CSL | (1 of 1) PTHR23155//PTHR23155:SF444 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Chlamydomonas SHOC2/SUR8-like LRR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTCTATATCTCTGCCCTCTCTCCTTCA |
| Internal bar code: | GGAGTCCGATTCTCCGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.5449 |
| LEAP-Seq n confirming: | 3500 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACACATCCCTGCTCA |
| Suggested primer 2: | CAGGCTTACCCTGTGCTCTC |