| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187933 |
| Chromosome: | chromosome 11 |
| Location: | 1893149 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCACCCGCGCCGCCGCCACCGCCACTGC |
| Internal bar code: | GCTGTGTATCCTTCCTCGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCCTATGAAAGCACACG |
| Suggested primer 2: | GAATGGAAGGGAAGGGAAAG |