| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187933 |
| Chromosome: | chromosome 11 |
| Location: | 1893150 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCAGCGGCCCCAATGGCCGCGGCTCT |
| Internal bar code: | ACGTTGGGTGCGATGGGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 28.4465 |
| LEAP-Seq n confirming: | 5629 |
| LEAP-Seq n nonconfirming: | 14159 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCCTATGAAAGCACACG |
| Suggested primer 2: | GAATGGAAGGGAAGGGAAAG |