| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188027 |
| Chromosome: | chromosome 3 |
| Location: | 5543637 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186250 | (1 of 1) PTHR22935//PTHR22935:SF77 - PENICILLIN-BINDING PROTEIN // BETA-LACTAMASE FAMILY PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGACACCGTCTTCAGAGGCCCATGTTCT |
| Internal bar code: | CACGAGCTCATTGATTAGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.061 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAGCATTTGATGGGGAG |
| Suggested primer 2: | CACAGCGCTTTCGTTATTGA |