Insertion junction: LMJ.RY0402.188034_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188034
Chromosome:chromosome 16
Location:1159459
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g650425 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGACATACACACAAGTCCAATAACAGTT
Internal bar code:CCGATCAGAGGCGCGTTGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:81.1024
LEAP-Seq n confirming:1339
LEAP-Seq n nonconfirming:312
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCTTGGATCCTGACACG
Suggested primer 2:GGGTAGCATGATTGCGGTAT