Insertion junction: LMJ.RY0402.188053_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188053
Chromosome:chromosome_3
Location:5077184
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g181150 DLL1,ROC106,FLA14,RSP22,LC8 Dynein arm light chain 8, LC8 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):AGAAGGAGTTCGACCGGAAGCACAACCCGA
Internal bar code:TTGCGTATCCTGAACCCAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:769
LEAP-Seq percent confirming:79.9248
LEAP-Seq n confirming:4037
LEAP-Seq n nonconfirming:1014
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTTCCTCCCCTTCCTTC
Suggested primer 2:AAATGTATTTCGACCCTGCG