| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188068 |
| Chromosome: | chromosome 8 |
| Location: | 4843808 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384600 | (1 of 2) 3.1.1.81 - Quorum-quenching N-acyl-homoserine lactonase / Quorum-quenching N-acyl homoserine lactone hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTACTCAATCCCAACGAAACCGGGGAT |
| Internal bar code: | TTGGGGTATGTGAACAGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 84.1362 |
| LEAP-Seq n confirming: | 1013 |
| LEAP-Seq n nonconfirming: | 191 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCTCAAGCTATTCCC |
| Suggested primer 2: | ACGTCCGCACCATAACTACC |