| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188078 |
| Chromosome: | chromosome 8 |
| Location: | 3713324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCATCGGCCCCAGTCGTGTGCAAGCACA |
| Internal bar code: | CGCGGGCTGGATCAATCCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1038 |
| LEAP-Seq percent confirming: | 98.6086 |
| LEAP-Seq n confirming: | 6024 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCGTTTCCTTGGGATTG |
| Suggested primer 2: | AAGAAGCGCTGAGAAAGCTG |