| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188081 |
| Chromosome: | chromosome 6 |
| Location: | 7805098 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302551 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAATCAATCGATCGCTGAAATAATTAA |
| Internal bar code: | ACGGCTATCCGGCCTGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGCATAGTGTTTCGAC |
| Suggested primer 2: | TAACTGCCAGACACGACAGG |