| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188090 |
| Chromosome: | chromosome_3 |
| Location: | 2940669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g163200 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTACGACCTCATATTTGCTGTGGGCCCTG |
| Internal bar code: | CCACGCTGCCTGGTTCCAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.841 |
| LEAP-Seq n confirming: | 3768 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCTGATGGGCTAGCAGT |
| Suggested primer 2: | CGGATTTGGTGGAAGAGGT |