| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188090 |
| Chromosome: | chromosome_13 |
| Location: | 235462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g563700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGGCCTGTGCAAGTGAAGGCAAGCAACAC |
| Internal bar code: | GCCACTCCGGTATGCTGGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 98.7805 |
| LEAP-Seq n confirming: | 162 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCCGCTTGTCGTTTTAC |
| Suggested primer 2: | GAACAAGACTGAAGACCGCC |