| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188138 |
| Chromosome: | chromosome 12 |
| Location: | 7178781 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558650 | EIF2B,EIF2BD | (1 of 1) K03680 - translation initiation factor eIF-2B subunit delta (EIF2B4); eIF-2B delta subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAAAGGTGTGGTTCGCCGCCCTGCTCTG |
| Internal bar code: | CCATGCGAACTGCGGGGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 96.7705 |
| LEAP-Seq n confirming: | 2547 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTCGGAGATATGGGAAC |
| Suggested primer 2: | TCAGTGGAGTTGAGCGACAC |