| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188139 |
| Chromosome: | chromosome 17 |
| Location: | 2898220 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719701 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTATATCCTCTCTCTGCGGTCCCGTCCT |
| Internal bar code: | GGGTAACGCCTTGTAAGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.4646 |
| LEAP-Seq n confirming: | 2415 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCGTAATTCTTGCTCG |
| Suggested primer 2: | CAGTGGTTTCGGCCTACAAT |