| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188169 |
| Chromosome: | chromosome 10 |
| Location: | 2515357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436400 | (1 of 2) PTHR13271//PTHR13271:SF11 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCACACATGCGCATCTCAGCGCCACTCA |
| Internal bar code: | CACACCGGCCCGATCTAATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1142 |
| LEAP-Seq percent confirming: | 99.0805 |
| LEAP-Seq n confirming: | 2155 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACAGCATGGTACGTGA |
| Suggested primer 2: | TCTGCTGTTACGGTACTGCG |