| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188211 |
| Chromosome: | chromosome 1 |
| Location: | 6268350 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044850 | (1 of 1) K17592 - sacsin (SACS) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCTTGCACTGCCACGCGGAGCGAGCGT |
| Internal bar code: | GCTTAGTTGGGGGGGCTCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 87.3596 |
| LEAP-Seq n confirming: | 933 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGTGTGCAAACTTCGTA |
| Suggested primer 2: | TACCGTGTGCTTGTGGAGAC |