| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188211 |
| Chromosome: | chromosome_1 |
| Location: | 6268353 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g044850 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTGTTTCAGCCGCGACGTGGATGCCGCAT |
| Internal bar code: | CGGGGAATCGCTGGCGGTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.87 |
| LEAP-Seq n confirming: | 2304 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTGTGCTTGTGGAGAC |
| Suggested primer 2: | GAGCGTGTGCAAACTTCGTA |