| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188220 |
| Chromosome: | chromosome 8 |
| Location: | 3499957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein 86 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAAGCCGACGTGTGCGCAGTCACTAGT |
| Internal bar code: | TTGGCTCTAGGTTTTCTATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.4732 |
| LEAP-Seq n confirming: | 4532 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTGGAGCCGATGAGCTT |
| Suggested primer 2: | TTCGAGTTGTCATTTGAGCG |