Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.188220 |
Chromosome: | chromosome_8 |
Location: | 3499957 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g375050 | FAP86 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCGAAGCCGACGTGTGCGCAGTCACTAGT |
Internal bar code: | TTGGCTCTAGGTTTTCTATTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 494 |
LEAP-Seq percent confirming: | 99.4732 |
LEAP-Seq n confirming: | 4532 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTTGGAGCCGATGAGCTT |
Suggested primer 2: | TTCGAGTTGTCATTTGAGCG |