Insertion junction: LMJ.RY0402.188326_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188326
Chromosome:chromosome 12
Location:1093875
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g490750 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCATTGCACGCCTTGATCACACACATCA
Internal bar code:GTGAAAGGGAAGGGCACCGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:695
LEAP-Seq percent confirming:97.973
LEAP-Seq n confirming:290
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGATTGGTGTCTGACG
Suggested primer 2:ACACAGGGGACCTGAAACAG