| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188358 |
| Chromosome: | chromosome_11 |
| Location: | 2047427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g468550 | ATPVG,ATPvG | Vacuolar ATP synthase subunit G | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGAGCGAAAGTTGCCCTTGTCGACTTCCG |
| Internal bar code: | TCGGTGACAGAACGCACTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.2727 |
| LEAP-Seq n confirming: | 273 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCAACAACACGATG |
| Suggested primer 2: | TCACGTGCTTCAATTTCTGC |