Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.188358 |
Chromosome: | chromosome_12 |
Location: | 3431852 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g496500 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACATTCCATCAGGCTCCAAAATTCAGGCC |
Internal bar code: | CGGGCTCTCGAAGGAGAGAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 505 |
LEAP-Seq percent confirming: | 98.8996 |
LEAP-Seq n confirming: | 719 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAATTTGTTGCCGTGTGATG |
Suggested primer 2: | ACGTACTGGATGAAGGGTGC |