| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188388 |
| Chromosome: | chromosome 3 |
| Location: | 4082028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172800 | (1 of 1) K18753 - butyrate response factor 1 (ZFP36L) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGGGAATTCACTGACCCCAGACTCCA |
| Internal bar code: | AGATGAGAAGGAGTATCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 95.935 |
| LEAP-Seq n confirming: | 236 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTCTCGCTTACGAAGG |
| Suggested primer 2: | CACGGGAGCTACAGGAAGAG |