Insertion junction: LMJ.RY0402.188425_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188425
Chromosome:chromosome 5
Location:2943313
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAAGAGCGAGGAGGAGTGGGGGAAATAG
Internal bar code:CGGTGCGCTTTCCTGCACGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:99.8743
LEAP-Seq n confirming:3178
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCTGTCCCTGTCCCT
Suggested primer 2:AACCAAGCTCAAGTAGCGGA