| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188431 |
| Chromosome: | chromosome 1 |
| Location: | 5910694 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGGTTCGAGTCCAGCAGAGAGGTGAAG |
| Internal bar code: | TGCCGAACTCCAGCTGGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 98.4707 |
| LEAP-Seq n confirming: | 1159 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTGCTATTGTCGCGGCT |
| Suggested primer 2: | GAGCGGGAGTTTATGATGGA |