| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188475 |
| Chromosome: | chromosome 7 |
| Location: | 4057724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340350 | MASAP,P60,ASA1 | Mitochondrial F1F0 ATP synthase associated protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACACACTGTTGCTCTCCCAGAGCCAGC |
| Internal bar code: | GTCAAGTGCGCCCTACTAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.6005 |
| LEAP-Seq n confirming: | 2493 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCAGGACTTCGACATCA |
| Suggested primer 2: | TAGCATGCTCATCCCCCTAC |