| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188482 |
| Chromosome: | chromosome 16 |
| Location: | 2017777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656900 | (1 of 77) IPR001623 - DnaJ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAGGTAACGAGTGCGGCCGGTCTTTCC |
| Internal bar code: | GACGTCGCCGGTCCGATGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 93.5211 |
| LEAP-Seq n confirming: | 664 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTATGAACACACGGTTT |
| Suggested primer 2: | GAAGGGAGCGTAATCGACAG |