| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188511 |
| Chromosome: | chromosome 6 |
| Location: | 5154130 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281400 | NAH1 | Cation antiporter; (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGCCCTTGCGGTCCGCCCATTTGAGGA |
| Internal bar code: | TCCTTTACGGGATACCGAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.8335 |
| LEAP-Seq n confirming: | 1799 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTATGTGTGTGGTGGAAC |
| Suggested primer 2: | GCACATGACCACAAGACCAC |