Insertion junction: LMJ.RY0402.188562_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188562
Chromosome:chromosome 3
Location:5524556
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g186050 (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGAGCCAATGTTCCGGTTAGCTGGACAG
Internal bar code:GCGCACACGTGATCTATCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:96.139
LEAP-Seq n confirming:249
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGACCGGCACAAGTTATT
Suggested primer 2:AAATGGTTCTGGTCCGTCTG