| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188571 |
| Chromosome: | chromosome 6 |
| Location: | 255762 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250750 | (1 of 1) K11842 - ubiquitin carboxyl-terminal hydrolase 12/46 [EC:3.4.19.12] (USP12_46) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGATGCTGCCCTGAGCGTGCACGAGTGG |
| Internal bar code: | GTTGGCGTCCCGCGTTCACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 95.6137 |
| LEAP-Seq n confirming: | 7019 |
| LEAP-Seq n nonconfirming: | 322 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAACTGCCTGCGTATCAG |
| Suggested primer 2: | TCTGCAGTTGGCCCTCTACT |