| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188609 |
| Chromosome: | chromosome 16 |
| Location: | 1354626 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651850 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTGTCGCAGAGGTCAGCAGAGTGGCAG |
| Internal bar code: | GCATATGGGGGGCGTGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.6903 |
| LEAP-Seq n confirming: | 2253 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTTGGTTATCAGCTCG |
| Suggested primer 2: | GTGACGGCGACTTCTTCTTC |